Atp4 guidelines 2013 pdf

Nevertheless, our results provided a strong evidence that optimal RPS8 expression contributes to cold-adaptability in rice. The wild-type background of this line is the cv Dongjin. C and W are two rice accessions belonging to the aus subpopulation Zhao et al. Agronomic trait analyses were performed by growing wild-type and mutant plants under natural field conditions at the experimental station of Huazhong Agricultural University, Wuhan, China For transgene complementation, a 5,bp wild-type genomic DNA fragment containing the entire OsATP4 coding region with a 3,bp upstream region and a bp downstream sequence was amplified using the primers Pcom-F and Pcom-R Supplemental Table S2.

The primers incorporated a Kpn I site at the N-terminal end and the C-terminal end. Nucleotide sequences were analyzed with Sequencher software Gene Codes Corporation. Chlorophyll content was measured using the method described by Arnon The leaves of two-leaf seedlings were harvested, weighed, and finely ground in liquid N 2. Total chlorophyll was extracted with a mixture of ethanol, acetone, and water 4. Absorption values at and nm were recorded to calculate the chlorophyll content.

For the chlorophyll fluorescence measurement, the seedlings were incubated in the dark for 30 min and subsequently placed in a kinetic imaging fluorometer model no. The plasmid was transformed into protoplasts obtained from green rice tissue as described in Zhang et al. Protoplasts were incubated overnight at room temperature in the dark, and the fluorescence signal was observed with a confocal microscope Leica.

A model no. The cDNA above was used as the template. The amplified PCR products were directly sequenced by Sangon Biotech, and the results were compared with the corresponding DNA sequences for each transcript. Southern blot analysis was performed with the probe labeled by digoxigenin DIG.

Prehybridization, hybridization, and chemiluminescent detection were performed following the DIG application manual provided by Roche Diagnostics. Total proteins of osatp4 and wild-type cv Dongjin seedlings were isolated with an extraction buffer containing 50 m m of Tris-HCl at pH 8.

The protein concentration was determined by the Bradford method Bradford, Bovine serum albumin was used as the standard. The subsequent immunodetection was performed following a standard protocol for the primary antibody.

The following supplemental data are available. Supplemental Figure S1. Identification of cr - osatp4 and rps8 editing defects in cr - osatp4. Supplemental Figure S2. Supplemental Figure S3. RNA editing of rps8 - in ppr - 1. Supplemental Figure S4. Supplemental Figure S5.

RNA gel blot hybridization of rpl16 transcripts in C Supplemental Figure S6. Accumulation of rRNAs in wild type and osatp4. Supplemental Table S1. Supplemental Table S2. List of oligonucleotides used in this study. Arnon DI Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol 24 : 1 — Google Scholar. Barkan A Expression of plastid genes: Organelle-specific elaborations on a prokaryotic scaffold.

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Nucleic Acids Res 31 : — Oxford University Press is a department of the University of Oxford. It furthers the University's objective of excellence in research, scholarship, and education by publishing worldwide. Sign In or Create an Account. Sign In. Advanced Search. Search Menu. Article Navigation. Close mobile search navigation Article Navigation. Volume Article Contents Abstract.

Jinghong Zhang , Jinghong Zhang. Oxford Academic. Yipo Guo. Qian Fang. Yongli Zhu. Yang Zhang. Xuejiao Liu. Yongjun Lin. Alice Barkan. Fei Zhou. Select Format Select format. Permissions Icon Permissions.

Abstract Chloroplast gene expression involves the participation of hundreds of pentatricopeptide repeat PPR RNA binding proteins, and proteins in the PLS subfamily typically specify sites of RNA editing, whereas those in the P-subfamily typically stabilize RNA, activate translation, or promote intron splicing.

Open in new tab Download slide. Table 1. Agronomic performance of wild-type and osatp4 plants. Plant Height. Tillers per Plant. Panicle Length. Filled Grain Rate. Yield per Plant. Open in new tab. Table 2. Wild Type. Copper enzymes in isolated chloroplasts. Google Scholar Crossref. Search ADS. Expression of plastid genes: Organelle-specific elaborations on a prokaryotic scaffold. A combinatorial amino acid code for RNA recognition by pentatricopeptide repeat proteins.

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Development of novel glyphosate-tolerant Japonica rice lines: A step toward commercial release. Google Scholar PubMed. The revised version of the manuscript is being reviewed by the ILAE and will then be submitted for publication. Review proposed guidelines and comments. Review proposed guideline and comments.

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